What is the function of the acid glycoprotein assay kit

The core function of the Acid Glycoprotein Assay Kit (mainly for measuring alpha 1-acid glycoprotein, AAG) is to quantitatively detect the concentration of AAG in serum or plasma, assist in clinical diagnosis of diseases such as inflammation, infection, tumors, and liver disease, and guide drug dosage adjustment and efficacy monitoring. The following is an explanation from three aspects: principle of action, clinical application, and detection value:

1. Principle of action: quantitative detection of AAG concentration

The acid glycoprotein assay kit is based on immunological principles (such as immunoturbidimetry, enzyme-linked immunosorbent assay, chemiluminescence immunoassay, etc.), and quantitatively determines the concentration of AAG in the sample through antigen antibody specific binding reaction. For example:

Immunoturbidimetry: AAG in the sample binds with anti AAG antibodies to form an immune complex, and the AAG content is calculated by measuring the changes in the absorbance of the complex.

Enzyme linked immunosorbent assay (ELISA): Using a double antibody sandwich method, AAG capture antibodies are coated on a microplate, added to the sample, and then combined with HRP labeled detection antibodies to determine AAG concentration through colorimetric reaction.

. Chemiluminescent immunoassay (CLIA): using chemiluminescent substrates (such as luminol) to emit light under enzyme catalysis, AAG concentration is calculated by measuring the luminescence value, which has high sensitivity and long luminescence duration.

II. Clinical application: auxiliary diagnosis and monitoring of various diseases

AAG, as an acute phase reactive protein, has significant changes in its concentration under pathological conditions such as inflammation, infection, tumor, liver disease, etc. Its detection is widely used in clinical practice:

AAG is an early sensitive inflammatory marker, whose rise is earlier than changes in body temperature and white blood cell count, and is widely used in auxiliary diagnosis of infectious diseases. Example: In sepsis, respiratory tract infection, intestinal infection, and central nervous system infection, serum AAG levels significantly increase and decrease after the condition improves.

Tumor monitoring

Serum AAG concentration in patients with malignant tumors (such as liver cancer, lung cancer, breast cancer) increased, especially when cancer metastasized.

Example: Dynamic observation of AAG levels is helpful for tumor recurrence monitoring and efficacy evaluation, such as in the remission period of acute leukemia patients with AAG close to the normal group.

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Liver Disease Assessment

AAG is synthesized by the liver, and its synthesis decreases when the liver parenchyma is damaged, resulting in a decrease in blood levels.

. Example: The serum AAG concentration in patients with chronic liver disease decreases, and the more severe the condition, the more obvious the decrease. It can be used as an indicator of liver reserve function and prognosis in patients with cirrhosis.

Medication dose adjustment

AAG is the main binding protein of various drugs (such as lidocaine, propranolol, paclitaxel), and its concentration changes affect drug metabolism.

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Example: The initial AAG level in gynecological cancer patients is associated with the toxic side effects of paclitaxel drugs. Patients with low AAG are more prone to blood toxicity and need to adjust the dosage.

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III. Detection value: Provides objective diagnostic basis

Differential diagnosis

Changes in AAG levels can assist in distinguishing acute phase reactions from protein level elevation caused by estrogen (the latter having normal or decreased AAG concentration).

. Combined with binding globulin assay, intravascular hemolysis can be distinguished (elevated AAG and normal binding globulin indicate mild hemolysis).

Efficacy monitoring

Dynamic observation of changes in AAG levels can help evaluate treatment efficacy.

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Example: The level of AAG in patients with secondary infection of diabetes was significantly higher than that in the non infection group. AAG decreased after anti infection treatment, indicating the efficacy.

Prognostic assessment

AAG levels are correlated with disease severity and prognosis.

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Example: The serum AAG level in children with acute pneumonia increases as the condition worsens, and can be used as an important reference indicator for the severity and prognosis of the disease.

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