Precautions for using the B-factor assay kit

The precautions for using the B-factor assay kit are as follows:

Kit temperature recovery and storage:

After removing the kit from the refrigerated environment, it should be equilibrated at room temperature for 15-30 minutes before use to ensure stable reagent performance. When storing, avoid direct sunlight and store in a dry, shaded, and suitable temperature place (such as a low-temperature refrigerator between -20 ℃ and 70 ℃) to prevent reagent failure.

Preparation and use of reagents:

If there is crystallization in the concentrated washing solution, it can be dissolved by heating in a water bath, diluted before use, and it is necessary to ensure that the washing solution fully covers the bottom of the well. After each washing, pat dry the liquid.

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Each step of sample addition should use a sample applicator and calibrate its accuracy. The sample addition time should be controlled within 5 minutes, and it is recommended to use a sampling gun to improve efficiency.

. The sealing film is only for one-time use to avoid cross contamination; The substrate should be stored away from light to prevent abnormal color development.

Sample processing and preservation:

Serum samples should be left at room temperature for 2 hours or overnight at 4 ℃ before centrifugation to obtain the supernatant, or directly stored at -20 ℃ to -80 ℃, but repeated freezing and thawing should be avoided.

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Plasma samples can be anticoagulated with EDTA or heparin, and centrifuged within 30 minutes after collection. Similarly, repeated freezing and thawing should be avoided.

. Cell culture supernatant, tissue homogenate and other samples should be processed according to the instructions to ensure that there are no particles or polymers interfering with the detection.

Experimental operating procedures:

Strictly follow the instructions, and the judgment of experimental results must be based on the reading of the enzyme-linked immunosorbent assay reader to avoid subjective judgment.

. Different batches of reagents should not be mixed to avoid affecting the accuracy of results due to differences in composition.

The Flat noodles not used in the experiment should be immediately put back into the packaging bag for sealing and preservation to prevent deterioration.

Safety protection and waste disposal:

When using chemical reagents, protective equipment should be worn to avoid direct contact with the termination solution and substrates A and B. Once in contact, rinse immediately with water.

. All samples, detergents, and waste must be treated as infectious agents to prevent environmental pollution and cross infection.

Special handling and precautions:

Avoid heat quenching the complement components in the blood activating serum. Unless necessary, heat treatment is not recommended to avoid affecting serum quality.

. If the content of the substance to be tested in the sample is too high, it needs to be diluted with a sample diluent before measurement, and the calculation should be multiplied by the total dilution factor. After the experiment is completed, the OD value should be read immediately to avoid color changes affecting the accuracy of the results.