What are the characteristics of the fibrinogen assay kit

The fibrinogen assay kit is mainly used for quantitative detection of fibrinogen content in samples (such as serum, plasma, etc.) in vitro. Its characteristics are reflected in the detection method, sensitivity and specificity, repeatability, reagent composition and stability, ease of operation, and application scope. The following is a specific introduction:

Detection method

The double antibody one-step sandwich method in enzyme-linked immunosorbent assay (ELISA) is adopted, which has high accuracy and reliability.

Sensitivity and specificity

High sensitivity: able to detect lower concentrations of fibrinogen, meeting the precise detection needs of scientific research and clinical practice.

. For example, some test kits have a minimum detection concentration of less than 0.1ng/mL or 0.1U/L. Strong specificity: does not cross react with other soluble structural analogues, ensuring the accuracy of detection results.

Repeatability

Good repeatability: The intra plate coefficient of variation and inter plate coefficient of variation are both small, ensuring the stability and reliability of the test results.

. For example, the intra plate coefficient of variation of some test kits is less than 10%, and the inter plate coefficient of variation is less than 15%.

Reagent composition and stability

Reagent composition is clear: usually includes coated micropores with human fibrinogen capture antibodies, standard samples, HRP labeled detection antibodies, substrate TMB, termination solution, etc.

Good stability: Under specified storage conditions (such as 2-8 ℃), the reagent kit has a longer shelf life and is easy to store and use.

. For example, some test kits have an expiration date of 6 months.

Easy to operate

Easy to operate: The reagent kit provides detailed experimental steps and instructions, and users only need to follow the steps to complete the detection.

. Meanwhile, the reagents and equipment in the kit are disposable, avoiding the risk of cross contamination.

Scope of application

Widely used: suitable for in vitro quantitative detection of human fibrinogen content in serum, plasma, tissue homogenates, and related liquid samples.

. In the field of scientific research, it can be used to study the relationship between fibrinogen and diseases; In the clinical field, it can be used to assist in the diagnosis of certain diseases related to abnormal fibrinogen.