What are the characteristics of the B-factor assay kit

The B-factor assay kit, as an in vitro diagnostic tool, is mainly used for detecting or quantitatively analyzing B-factor (complement factor B). Its characteristics are reflected in multiple dimensions such as technical performance, operating experience, application scope, and quality assurance. Specifically, it is as follows:

1. Technical performance: high sensitivity and strong specificity

Outstanding sensitivity

Using enzyme-linked immunosorbent assay (ELISA) or latex enhanced immunoturbidimetry, it can detect B-factor content as low as 0.1ng/ml, suitable for early disease screening or disease monitoring. For example, in patients with systemic lupus erythematosus (SLE), the level of factor B may only slightly increase, and a high-sensitivity kit can accurately capture this change.

Excellent specificity

Through antigen antibody specific binding, the kit can accurately distinguish factor B from other similar proteins (such as C3b), reducing misdiagnosis caused by cross reactivity.

. For example, in the diagnosis of hereditary angioedema (HAE), a highly specific kit can avoid misjudgment due to abnormalities in other complement components.

Reliable accuracy

Combined with precise experimental design (such as double antibody sandwich method) and quality control system, the kit provides reliable detection results.

. For example, in patients with paroxysmal nocturnal hemoglobinuria (PNH), the level of factor B is closely related to disease activity, and highly accurate test kits can provide a basis for treatment decisions.

2. Operating Experience: Simple, Efficient, and Repeatable Stability

Simplified Operating Process

The reagent kit uses ready to use liquid dual reagents or pre coated enzyme-linked immunosorbent assay plates to reduce experimental steps.

. For example, ELISA kits usually only require four steps: sample addition, incubation, washing, and color development, which can be quickly mastered by experimenters and improve work efficiency.

Excellent repeatability

Through strict quality control (such as batch difference ≤ 5%), the reagent kit can obtain stable results under different batches and experimental conditions.

. For example, in long-term monitoring of factor B levels in SLE patients, a well reproducible kit can ensure data consistency and provide reliable basis for disease assessment.

Intuitive result determination

Using colorimetric methods (such as OD value determination in ELISA) or turbidity methods (such as absorbance changes in immunoturbidimetric methods), the result determination is intuitive and quantifiable.

. For example, in the treatment of atypical hemolytic uremic syndrome (aHUS), regular monitoring of factor B levels can provide a visual assessment of drug efficacy.

III. Application scope: Multi scenario coverage and multi species applicability

Widely applicable for clinical diagnosis

Suitable for auxiliary diagnosis and disease monitoring of complement system related diseases such as hereditary angioedema (HAE), paroxysmal nocturnal hemoglobinuria (PNH), systemic lupus erythematosus (SLE), etc.

. For example, in SLE patients, excessive activation of factor B may exacerbate inflammatory responses, and detecting its levels can help evaluate disease activity.

In depth research in the field

Support complement system function research, drug development, and gene editing animal validation.

. For example, studying the role of factor B in complement overactivation in severe COVID-19 patients can provide a basis for treatment strategies.

Multi species compatibility

Not only suitable for human samples, but also for B-factor determination in animal samples (such as sheep, chickens, ducks, fish, etc.).

. For example, in agricultural research, detecting the level of factor B in fish can evaluate their immune status and optimize aquaculture management.

Fourth, Quality Assurance: Strict System and Perfect Service

Strict Quality Control

Well known brands (such as Sigma and Enzyme linked Biotech) have passed international certifications such as ISO 13485 for their reagent kits, ensuring full process quality control from raw materials to finished products.

. For example, using imported antibodies with original packaging ensures uniform adsorption, high recycling rate, and strong reliability.

Convenient storage and transportation

Reagent kits are usually stored in an environment of 2-8 ℃, with a long shelf life (such as 12-18 months), making them easy to store for a long time.

. The transportation conditions are simple, and conventional methods such as express delivery can be used to reduce logistics costs.

Complete after-sales service

Provide services such as return and exchange, free testing, etc., to solve users' worries.

. For example, if quality issues are found, users can promptly contact the manufacturer to replace the reagent kit to ensure that the experimental progress is not affected.