Materials for the production of B-factor assay kit

The materials used to make the B-factor assay kit vary depending on the detection method. The following are two common types of kit materials:

Enzyme linked immunosorbent assay (ELISA) kit

Enzyme linked immunosorbent assay (ELISA) plate: usually 96 or 48 wells, used to immobilize antibodies.

Standard: Contains a known concentration of factor B, used to plot the standard curve.

. Sample diluent: used to dilute the sample to be tested.

Detection antibody HRP: a horseradish peroxidase labeled detection antibody used to bind to factor B in the sample.

. Wash buffer: used to wash unbound substances and reduce background interference. Substrate solution: such as TMB (tetramethylbenzidine), which develops color under the catalysis of HRP. Termination solution: such as sulfuric acid or citric acid, used to terminate the color reaction. Sealing film: used to cover the enzyme-linked immunosorbent assay (ELISA) plate to prevent evaporation and contamination.

Instruction manual: Provide instructions and precautions for using the reagent kit.

. Other auxiliary materials: such as self sealing bags, high-precision samplers, and gun heads.

Immunoturbidimetric assay kit

Reagent R1:

Tris HCl buffer solution: provides a suitable pH environment.

. Sodium chloride (NaCl): maintains osmotic pressure. Polyethylene glycol (PEG): As a turbidity enhancer, it improves detection sensitivity. Sodium azide (NaN3): As a preservative, it prevents the reagent from deteriorating.

Reagent R2:

Tris HCl buffer solution: also provides a suitable pH environment.

. Sodium chloride (NaCl): maintains osmotic pressure. Anti human factor B antibody: binds to factor B in the sample to form an antigen antibody complex. Sodium azide (NaN3): used as a preservative.

Calibration standard:

Phosphate buffer solution: provides a suitable pH environment.

. Factor B antigen: used to calibrate the detection range of the kit. Biological preservatives, such as P-300, prevent the deterioration of calibration samples. Sucrose: As a stabilizer, it improves the stability of calibration standards.

Quality control product:

The composition is similar to the calibration product, but the concentration of factor B antigen is different. It is used to monitor the detection accuracy of the reagent kit.

.