Storage method of ischemia modified albumin assay kit

The storage method of the ischemia modified albumin assay kit should be standardized according to the status of the kit, the type of specimen, and the specific components, as follows:

1. Storage of unopened kit

Overall storage conditions: All reagents should be stored according to the label on the reagent bottle. It is usually recommended to store the standard, detection solution A, detection solution B, and 96 well plate at -20 ℃ to ensure the stability and activity of the reagents.

Validity management: The expiration time of the product is based on the label on the box, and all components are ensured to be stable within the shelf life.

. The expiration date of the reagent kit is usually 6 months, but please refer to the product manual for details.

2. Storage of the reagent kit after use

Storage of remaining reagents: After use, the remaining reagents of the reagent kit still need to be stored at the temperature indicated on the label of the reagent bottle.

. After opening, the enzyme-linked immunosorbent assay (ELISA) plate should be sealed with a desiccant and stored at -20 ℃ to avoid moisture.

Suggestions for repeated use: The enzyme-linked immunosorbent assay (ELISA) strip in the kit can be disassembled and used multiple times according to experimental requirements.

. It is recommended to use the remaining reagent kits within one month after the first experiment to ensure the quality of the reagents.

III. Specimen Preservation

Serum specimens:

After collection, they can be left at room temperature for 2 hours or overnight at 4 ℃, then centrifuged at 1000 × g for 20 minutes, and the supernatant can be taken for detection.

. If storage is required, the supernatant can be placed at -20 ℃ or -80 ℃, but repeated freezing and thawing should be avoided to avoid affecting the test results.

Plasma specimens:

Use EDTA or heparin as anticoagulants to collect specimens, and centrifuge at 2-8 ℃ and 1000 × g for 15 minutes within 30 minutes after collection. The supernatant can be collected for detection.

. If storage is required, the supernatant can also be placed at -20 ℃ or -80 ℃ to avoid repeated freezing and thawing.

Other specimens:

Cell culture supernatant, tissue homogenate and other specimens need to be centrifuged at 1000 × g for 20 minutes to obtain the supernatant, then stored at low temperature and avoid repeated freezing and thawing.

.

Hemolytic specimens are not suitable for this test, as hemolysis can affect the final test results.

.

4. Storage of special components

Washing buffer: Concentrated washing solution may have crystal precipitation. When diluting, it can be dissolved by heating in a water bath, and washing does not affect the results.

. Diluted washing buffer should be used as needed to avoid waste.

Substrate solution: The substrate solution should be stored away from light. The substrate solution that has turned blue cannot be used because it indicates that the substrate has undergone a reaction and lost its ability to catalyze color development.

.

Fifth, Storage Precautions

Avoid contamination: Clean tips should be used when adding reagents and samples to prevent cross contamination.

. The sealing film is only for one-time use to avoid cross contamination.

Operating instructions: During incubation, ensure that the micropores are not dry and wash thoroughly to reduce background interference.

. Shake all liquid components thoroughly before use.

Safety precautions: Avoid direct contact with the termination solution and substrate solution during the experiment. If in contact, rinse immediately with water.

. Do not suck reagents with your mouth to ensure safe operation.