Scope of application of homocysteine assay kit

The homocysteine test kit is mainly used to quantitatively measure the total concentration of homocysteine (HCY) in human serum and plasma in vitro, and its application scope covers clinical detection, health assessment, disease risk prediction and other fields, specifically as follows:

I. Clinical test objects

chronic patient group

patients with cardiovascular and cerebrovascular diseases: The level of homocysteine (Hcy) is an independent risk factor for cardiovascular and cerebrovascular diseases, closely related to atherosclerosis and thrombosis.

Cancer patients: Research shows that Hcy is significantly increased in patients with breast cancer, liver cancer, stomach cancer, lung cancer and other malignant tumors, which can be used as a tumor marker for auxiliary diagnosis.

Patients with metabolic diseases: for example, patients with diabetes and endocrine diseases, the abnormal level of Hcy may reflect the degree of metabolic disorder. Kidney disease patients: The kidney is a key organ for Hcy metabolism, and decreased kidney function can lead to Hcy accumulation. Elevated blood levels are also independent risk factors for the progression of chronic kidney disease.

Patients with skeletal diseases: High blood levels can have a damaging effect on osteoblasts and osteoclasts, which may lead to decreased bone mass and bone strength.

People with chronic disease risk factors

Patients with hypertension, hyperglycemia, and hyperlipidemia: These populations often have elevated Hcy levels, which are associated with an increased risk of cardiovascular and cerebrovascular diseases.

People in special physiological stage

Pregnancy preparation population and pregnant women: Hyperglycemia can lead to pregnancy hypertension, diabetes, placental vascular disease and habitual abortion through vascular endothelial injury, embryonic development abnormalities and other mechanisms, and is also an independent risk factor for neural tube defects.

Health check-up population: used to assess overall health status and detect potential risks early.

2. Applicability of detection technology

Compatible with various biochemical analyzers

The reagent kit is suitable for semi/fully automatic biochemical analyzers, such as Hitachi series, Beckman series, Olympus series, Mindray series, Toshiba series, Abbott series, etc., to meet the equipment needs of different laboratories.

Scientific and reliable detection method

Enzyme cycling method: Accurate quantification is achieved through the reduction of oxidized Hcy, amplification of cycling reaction, and detection of NADH absorbance change rate.

III. Clinical significance and risk association

Risk of cardiovascular and cerebrovascular diseases

For every 5 μ mol/L increase in plasma Hcy, the risk of coronary heart disease increases by 32% and the risk of stroke increases by 59%.

Risk of diabetes nephropathy

The risk of diabetes nephropathy increased 3.86 times every 5 μ mol/L increase in plasma Hcy.

Progression of chronic kidney disease

High blood levels are negatively correlated with renal dysfunction and glomerular filtration rate. When blood levels are above 37.8 μ mol/L, the 5-year survival rate of kidney dialysis patients is less than 65%.

Risk of pregnancy related diseases

Pregnant women with blood equivalent values>8.59 μ mol/L have a 5.22-fold increased risk of giving birth to children with congenital heart disease.

Male diseases and fertility

High blood levels can lead to infertility by affecting sperm production and function, and patients with oligospermia/asthenozoospermia have significantly higher blood levels than healthy individuals.

Liver diseases

The liver is an important organ for Hcy metabolism, and liver cell damage can increase blood homocysteine levels, which in turn can exacerbate liver damage.