What are the advantages of the B-factor kit

As an in vitro diagnostic tool, the B-factor kit has significant advantages in terms of technical performance, operating experience, application scope, and quality assurance, as follows:

1. Technical performance advantages

High sensitivity

Using enzyme-linked immunosorbent assay (ELISA) or latex enhanced immunoturbidimetry, it can detect B-factor levels as low as 0.1ng/ml and is suitable for early disease screening or monitoring. For example, in patients with systemic lupus erythematosus (SLE), the level of factor B may only slightly increase, and a high-sensitivity kit can accurately capture this change. The optimized latex enhanced immunoturbidimetric method further enhances sensitivity and effectively detects factor B content by improving the latex preparation process, using composite buffer and sensitizer.

High specificity

Through antigen antibody specific binding, the kit can accurately distinguish factor B from other similar proteins (such as C3b), reducing misdiagnosis caused by cross reactivity.

. For example, in the diagnosis of hereditary angioedema (HAE), a highly specific kit can avoid misjudgment due to abnormalities in other complement components.

High accuracy

Combined with precise experimental design (such as double antibody sandwich method) and quality control system, the kit provides reliable detection results.

. For example, in patients with paroxysmal nocturnal hemoglobinuria (PNH), the level of factor B is closely related to disease activity, and highly accurate test kits can provide a basis for treatment decisions.

Strong anti-interference ability

In response to possible interfering substances such as lipid turbidity, bilirubin, rheumatoid factor, etc. in the sample, the kit adds a composite anti-interference agent (such as N-octadecyl-N - (2-sulfonyl) propyl-N, N-dimethyl betaine BSA、 Blockers, etc., effectively enhance anti-interference ability and ensure the stability of detection results.

2. Advantages of Operating Experience

Easy to operate

The reagent kit uses ready to use liquid dual reagents or pre coated enzyme-linked immunosorbent assay plates, reducing experimental steps.

. For example, ELISA kits usually only require four steps: sample addition, incubation, washing, and color development, which can be quickly mastered by experimenters and improve work efficiency.

Rapid detection

Most test kits have a short detection time (such as ELISA, which takes about 2 hours, and immunoturbidimetry, which is faster), and can quickly produce test results, meeting urgent clinical needs.

.

Intuitive results

Using colorimetric methods (such as OD value determination in ELISA) or turbidity methods (such as absorbance changes in immunoturbidimetric methods), the results can be judged intuitively and quantitatively.

. For example, in the treatment of atypical hemolytic uremic syndrome (aHUS), regular monitoring of factor B levels can provide a visual assessment of drug efficacy.

III. Advantages of Application

Clinical Disease Diagnosis and Monitoring

Suitable for auxiliary diagnosis and disease monitoring of complement system related diseases such as hereditary angioedema (HAE), paroxysmal nocturnal hemoglobinuria (PNH), systemic lupus erythematosus (SLE), etc.

. For example, in SLE patients, excessive activation of factor B may exacerbate inflammatory responses, and detecting its levels can help evaluate disease activity.

Research and drug development

Support complement system function research, drug development, and gene editing animal validation.

. For example, studying the role of factor B in complement overactivation in severe COVID-19 patients can provide a basis for treatment strategies; In drug development, it is used to verify the inhibitory effect of candidate compounds on factor B or bypass pathways.

Multi species Applicability

Not only applicable to human samples, but also for B-factor determination in animal samples (such as sheep, chickens, ducks, fish, etc.).

. For example, in agricultural research, detecting the level of factor B in fish can evaluate their immune status and optimize aquaculture management.

IV. Advantages of Quality Assurance

Strict Quality Control

Well known brands (such as Sigma and Enzyme linked Biotech) have passed international certifications such as ISO 13485 for their reagent kits, ensuring full process quality control from raw materials to finished products.

. For example, using imported antibodies with original packaging ensures uniform adsorption, high recycling rate, and strong reliability.

Stable reagent performance

By adding composite latex stabilizers (such as casein, gelatin, xanthan gum, etc.), the reagent kit can maintain the dispersion of latex particles, maintain antibody activity, prevent agglutination, and significantly enhance the stability of the reagent.

.

Comprehensive after-sales service

Providing services such as returns and exchanges, free testing, etc., to solve users' worries.

. For example, if quality issues are found, users can promptly contact the manufacturer to replace the reagent kit to ensure that the experimental progress is not affected.