What are the precautions for using the apolipoprotein C2 assay kit

The precautions for using the apolipoprotein C2 assay kit mainly include the following points:

Product usage and preparation before operation:

This product is only for external diagnostic use.

Before operation, it is necessary to carefully read the user manual and strictly follow the instructions in the reagent kit for experimental operations.

Experimental environment:

Avoid conducting experiments in harsh environments, such as those containing corrosive vapors and dust such as sodium hypochlorite, acid or alkali, or ether.

Reagent and sample processing:

After opening the wrapping strip, the remaining wrapping strip should be placed in a sealed bag to prevent moisture.

Micro pipette tips should not be mixed to avoid cross contamination.

Disposable gloves should be worn when handling reagents and samples, and hands should be thoroughly washed after operation.

All samples and used test kits should be considered as potential infectious substances, and should be disposed of in accordance with local government and national regulations.

Reagent usage and storage:

The reagent must be used within its validity period.

The remaining reagents should be sealed in a timely manner and stored at 2 ℃ to 8 ℃.

Precautions in the experimental steps:

When adding samples, it is necessary to ensure the accuracy of the sample amount and avoid the generation of bubbles.

The washing steps should be thorough, and the washing solution should be filled into each well, but excessive use of water should be avoided to prevent the formation of bubbles. Each wash should shake off the liquid inside the hole and pat it dry.

After adding luminescent substrates, the reaction should be carried out at room temperature in the dark, as strong light may affect the measurement results.

Strictly control the reaction time and temperature of each step, and the operation should be compact.

Result determination and data processing:

The result judgment should be based on the reading of the enzyme-linked immunosorbent assay (ELISA) reader.

If the OD value of the sample is higher than the upper limit of the standard curve, it should be diluted appropriately and retested, and multiplied by the corresponding dilution factor when calculating the sample concentration.

Other precautions:

If any problems are encountered during the experiment, such as incorrect dilution of standard samples, inaccurate pipetting, poor linearity of the standard curve, etc., the corresponding countermeasures in the instruction manual should be promptly referred to for handling.

If the experimental results are not good, please take photos of the color development results in a timely manner, save the experimental data, and contact technical support for assistance.

Please note that different brands or models of apolipoprotein C2 assay kits may have different specific operating steps and precautions. Therefore, it is important to carefully read and follow the instructions of the purchased kit before use.