Instructions for using the bilirubin assay kit

The conjugated bilirubin assay kit is mainly used for quantitative determination of conjugated bilirubin content in human serum in vitro, and is an important indicator for monitoring intrahepatic and extrahepatic bile stasis. The usage method may vary depending on the brand and model of the reagent kit, but generally speaking, the basic usage method of the conjugated bilirubin assay kit can refer to the following steps:

1、 Preparation work

Check reagents: Ensure that all reagents in the kit are within their expiration date and have not been contaminated or spoiled.

Prepare samples: Prepare an appropriate amount of serum sample according to the instructions of the reagent kit.

Preparation of instruments: Ensure that the required experimental instruments (such as spectrophotometers, micropipettes, etc.) have been calibrated and are in good working condition.

2、 Operation steps

Reagent preparation: According to the instructions of the reagent kit, mix reagent 1 and reagent 2 in a certain proportion to prepare the working solution. Note that different brands and models of reagent kits may require different preparation ratios and conditions.

Sample processing: Use a micropipette to accurately aspirate a certain amount of serum sample and add it to a reaction plate or test tube. Make sure not to mix the suction tips of micropipettes to avoid cross contamination.

Add working solution: Add an appropriate amount of working solution to the reaction plate or test tube containing the serum sample, and mix thoroughly.

Incubation: Place the reaction plate or test tube in a constant temperature incubator and incubate according to the temperature and time specified in the instructions.

Measurement: After incubation, use a spectrophotometer to measure the absorbance of the reaction solution at a specific wavelength (such as 450nm). Be careful to avoid strong light exposure during the measurement process to avoid affecting the measurement results.

Calculation: Calculate the concentration of conjugated bilirubin in the sample based on the measured absorbance and the calibration curve or equation provided by the reagent kit.

3、 Precautions

Operating standards: Strictly follow the requirements of the reagent kit instructions during the operation process to avoid inaccurate results caused by operational errors.

Safety protection: Disposable gloves should be worn when handling reagents and samples, and hands should be thoroughly washed after operation. All samples and used test kits should be considered potential infectious substances and disposed of in accordance with local government and national regulations.

Reagent storage: Unused reagents should be promptly sealed and stored under specified temperature conditions (such as 2 ℃~8 ℃) to avoid moisture or deterioration of the reagents.

Quality control: Quality control testing should be conducted during each experiment to ensure the accuracy and reliability of the experimental results.

Please note that the above steps are for reference only, and the specific usage method still needs to be carried out according to the instructions of the reagent kit. Different brands and models of reagent kits may have different operating methods and precautions, so it is important to carefully read the instructions and follow the instructions before use.