Introduction to the Function of Combined Bilirubin Determination Kit

The conjugated bilirubin assay kit is mainly used for in vitro quantitative determination of the concentration of conjugated bilirubin in human serum. Conjugated bilirubin, also known as direct bilirubin, is bilirubin in the blood that binds to glucuronic acid. The determination of its concentration is of great significance for clinical diagnosis and disease monitoring. The following is a detailed introduction to the function of the conjugated bilirubin assay kit:

1、 Main purpose

The main purpose of the conjugated bilirubin assay kit is to quantitatively determine the concentration of conjugated bilirubin in human serum. This test is of great significance for evaluating liver function, biliary system status, and monitoring the progression of certain diseases such as hepatitis, cirrhosis, biliary obstruction, etc.

2、 Working principle

Reagent kits are usually based on enzymatic or bilirubin oxidase methods for measurement. In enzymatic methods, the enzymes in the reagent kit can catalyze the conversion of conjugated bilirubin into a detectable substance, and indirectly reflect the concentration of conjugated bilirubin by measuring the concentration of the substance. In the bilirubin oxidase method, bilirubin oxidase can directly catalyze the oxidation reaction of conjugated bilirubin, producing quantifiable products that can be used to determine the concentration of conjugated bilirubin.

3、 Operation steps

Although the specific operating steps may vary depending on the brand and model of the reagent kit, they usually include steps such as sample collection, sample processing, reagent addition, reaction incubation, and result determination. Strictly follow the instructions of the reagent kit during operation to ensure the accuracy and reliability of the results.

4、 Clinical significance

Liver function assessment: The concentration of conjugated bilirubin can reflect the liver's metabolic capacity for bilirubin, thereby helping doctors evaluate the status of liver function.

Biliary system monitoring: Diseases such as biliary obstruction can cause obstruction in the excretion of conjugated bilirubin, leading to an increase in its concentration in the serum. Therefore, the determination of conjugated bilirubin is helpful in monitoring the status of the biliary system.

Disease diagnosis and differential diagnosis: The determination of conjugated bilirubin can also be used for the diagnosis and differential diagnosis of certain diseases, such as hepatitis, cirrhosis, biliary obstruction, etc.

5、 Precautions

Sample collection and processing: It should be ensured that the sample collection and processing process complies with regulations to avoid interference with the measurement results.

Reagent storage and use: The reagents in the reagent kit should be stored properly to avoid expiration or moisture damage. When using, strictly follow the instructions for operation.

Interpretation and judgment of results: The measurement results should be interpreted and judged based on clinical practice to avoid one-sided interpretation or misjudgment.

In summary, the bilirubin assay kit plays an important role in clinical diagnosis and disease monitoring, and its accuracy and reliability are of great significance for clinical decision-making.